She was the daughter of Vytautas the Great of Lithuania and his first wife, Anna. On 21 January 1391, while her father was engaged in the Lithuanian Civil War, she married Vasili I of Moscow. She was the longest serving consort of Russia.
After Vasili's death in 1425 she became regent for their ten-year-old son Vasili II. Her father supported Vasili's claim to the throne, which was disputed by his uncle, Yuri of Zvenigorod.
Yury Vasilievich (30 March 1395 – 30 November 1400)
Ivan Vasilievich (15 January 1396 or 1397 – 20 July 1417), died on the way from Kolomna to Moscow as a result of "pestilence", just six months after marrying the daughter of Prince Ivan Vladimirovich of Pronsk and receiving the inheritance of Nizhny Novgorod
Anastasia Vasilievna (d. 1470), wife of Vladymir Alexander, Prince of Kiev. Her husband was a son of Vladymir, Prince of Kiev. His paternal grandparents were Algirdas and Maria of Vitebsk.
Daniil Vasilievich (6 December 1400 – May 1402), died of pestilence
Vasilisa Vasilievna, wife of Alexander Ivanovich "Brukhaty", Prince of Suzdal, and Alexander Daniilovich "Vzmetenj", Prince of Suzdal.
Simeon Vasilievich (13 January – 7 April 1405), died of pestilence
Maria Vasilievna, wife of Yuri Patrikievich. Her husband was a son of Patrikas, Prince of Starodub, and his wife, Helena. His paternal grandfather was Narimantas.