Red fluorescent dye
Chemical compound
Sulforhodamine B or Kiton Red 620 (C27H30N2O7S2) is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells. This red solid dye is very water-soluble.[1]
Spectroscopy
The dye has maximal absorbance at 565 nm light and maximal fluorescence emission at 586 nm light.[1] It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.[2]
Applications
Sulforhodamine B is often used as a membrane-impermeable polar tracer[3] or used for cell density determination via determination of cellular proteins (cytotoxicity assay).[4]
References
- ^ a b "Sulforhodamine B sodium salt (CAS 3520-42-1)". Santa Cruz Biotechnology.
- ^ Coppeta, J.; Rogers, C. (1998). "Dual Emission Laser Induced Fluorescence for Direct Planar Scalar Behavior Measurements". Experiments in Fluids. 25: 1–15. doi:10.1007/s003480050202. S2CID 37649159.
- ^ Viricel W; Mbarek A; Leblond J (2015). "Switchable Lipids: Conformational Change for Fast pH-Triggered Cytoplasmic Delivery" (PDF). Angewandte Chemie International Edition. 54 (43): 12743–12747. doi:10.1002/anie.201504661. PMID 26189870. S2CID 24175578.
- ^ Vichai V; Kirtikara K (2006). "Sulforhodamine B colorimetric assay for cytotoxicity screening". Nature Protocols. 1 (3): 1112–1116. doi:10.1038/nprot.2006.179. PMID 17406391. S2CID 1245354.
