The Dutch Tweede Divisie in the 1963–64 season was contested by 32 teams, divided in two groups. NEC won the championship after beating Alkmaar '54 in a play-off.
Two teams would be promoted: the winners of the championship play-off and the winners of the following promotion competition. None of the teams relegated to amateur football.
Several play-offs were held to determine the league champions, who would be promoted to the Eerste Divisie, and who would leave the Professional leagues altogether.
NEC were promoted to the Eerste Divisie, while Alkmaar '54 entered the Promotion Tournament.
Roda JC qualified for the Best 3rd Place play-off owing to a superior Goal Average in the "regular season".
Roda JC entered the promotion tournament.
To determine the second team to be promoted. Entering teams:
However, as KFC & Be Quick 1887 both relegated themselves, LONGA retained their spot.