The 1999–2000 KNVB Cup was the 82nd edition of the tournament, at the time known as the Amstel Cup for sponsorship reasons. The competition started on 31 July 1999 and the final was played on 21 May 2000. Roda JC beat NEC 2–0 and received the cup for the second time. A total of 86 clubs participated.
The matches were played between July 31 and August 31, 1999. 79 clubs participated, 39 of which advanced to the next round.
E Eredivisie; 1 Eerste Divisie; A Amateur teams
The matches of the first round were played on September 22 and 23, 1999. sc Heerenveen entered the tournament this round, during the group stage they were still active in the Intertoto Cup.
E one Eredivisie entrant
The matches of the second round were played on October 28 and 30, 1999.
The matches of the round of 16 were played on January 27, 28 and 30, 2000. The Eredivisie teams that had been playing in European competitions after qualification last season, entered the tournament this round.
E six Eredivisie entrants
The quarter finals were played on February 16 and 17, 2000.
The semi-finals were played on April 11 and 12, 2000.
Roda JC would play in the UEFA Cup.