The first Duchess of Bohemia (česká kněžna) was St. Ludmila, while the first Queen of Bohemia (česká královna) was Świętosława of Poland. Some of them were (like their husbands) not crowned.
There was only one queen regnant in Czech history - Maria Theresa. Nevertheless, some female royal consorts were highly influential in the country's history, having ruled as regents for their minor children and heirs, as well as having a great influence over their spouses.
The title was used until 1918, when husband of the last queen was deposed.
House of Přemysl
Duchesses of Bohemia
874–888/891: Ludmila of Bohemia (Svatá Ludmila), wife of Bořivoj I, d. assassinated 15 September 921 in Tetín Castle
906–921: Drahomíra (princess Drahomíra ze Stodor), wife of Vratislav I, d. after 935
?–999: Emma of Melnik (Emma Regina or Hemma princess of Burgundy), original evident Queen of France, widow from King Lothair of France (d. 986), circa 989 second wife of Boleslaus I, d. 1005/1006